HEK Media Bundle

Our HEK Media Bundle is the easiest approach for you to test, identify and select the best matching serum-free, animal-component-free and chemically defined cell culture HEK media for your individual requirements and processes.

The HEK Media Bundle includes 1000 mL samples of up to 4 different liquid HEK cell culture media from Xell’s proprietary portfolio:

• HEK GM (order no. 851-0001)
• HEK ViP NX (order no. 892-0001)
• HEK ViP NB (order no. 891-0001)
• HEK TF (order no. 861-0001)

All of our media are designed as all-in-one solutions, from thawing to growth and production – no need for usage of several media. Xell products are available as liquid and dry powder formulations to support any process scale and related supply chain. 

• Suitable for HEK 293 and other human cell lines
• Chemically defined, serum-free, animal component-free and hydrolysate-free
• Little or no adaptation required from other serum-free media
• Support stable growth of suspension cells at high viability in seed train culture
• w/o L-glutamine
• Approved by ISO 9001 QMS (Quality Statement)

The transfection media (HEK ViP NX, HEK ViP NB and HEK TF) were developed and optimized for virus/viral vector production (e.g. AAV production and lentivirus production). They are especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI) and support cell growth and production phase of proteins or viral vectors in suspension culture in various scales. The media can be used in research or in large-scale GMP manufacturing applications. For more information on AAV-2 and AAV-8 production in HEK cells, please read our technical note.

Figure 1: Relative genomic titers (qPCR) in culture supernatant of different suspension HEK cell lines in their respective reference media compared to Xell’s HEK ViP NX media.

Figure 2: Left: Comparison of AAV-2 titers in supernatant vs. cells/pellets for processes with Xell's HEK ViP NX medium and two transfection reagents. Right: Optimization of processes leads to increased titer in culture supernatant.

Figure 3: Comparison of AAV8 genomic titers in supernatants of HEK293 cells cultivated in Xell's HEK ViP NX medium. Cultivation in bioreactor leads to increased genomic titer in culture supernatant.

 Please contact us and share with us any questions you might have.