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Culture Media Analysis Bundle

Culture Media Analysis Bundle

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Spent media analysis is the analysis of the used media over the course of the production... more
Product information "Culture Media Analysis Bundle"

Spent media analysis is the analysis of the used media over the course of the production process and facilitates the selection of the right cell culture medium and feed combination, as well as the development of suitable feeding strategies. It is a useful tool that enables us to gain insight into the metabolic processes as well as to track and understand changes in medium composition.

 

Xell's comprehensive culture media analysis bundle combines the most common and informative analytical parameters regarding spent cell culture medium. It includes the analysis of amino acids, glucose and lactate, trace elements and water-soluble vitamins.

Our proprietary, quantitative UHPLC-DAD method covers the following amino acids:
Alanine, Arginine, Asparagine, Aspartate, Citrulline, Cystine/Cysteine, Glutamate, Glutamine, Glycine, Histidine, Hydroxyproline, Isoleucine, Leucine, Lysine, Methionine, Ornithine, Phenylalanine, Proline, Serine, Taurine, Threonine, Tryptophane, Tyrosine, Valine

Glucose and Lactate are measured via an enzymatic-amperometric measurement.

Trace elements are determined by ICP-MS, combining highest sensitivity with excellent reliability, including:
Chromium (Cr), Manganese (Mn), Iron (Fe), Cobalt (Co), Nickel (Ni), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo), Cadmium (Cd)

Our proprietary UHPLC-MS/MS method covers the following water-soluble vitamins, vitaminoids and precursors:
Vitamin B1 Thiamine, Vitamin B2 Riboflavin, Vitamin B3 Nicotinamide, Vitamin B5 (Calcium) Pantothenate, Vitamin B6 Pyridoxal, Pyridoxine, Vitamin B7 Biotin, Vitamin B9 Folic acid, Aminobenzoic acid, Vitamin B12 Cyanocobalamin, Vitaminoids Choline chloride  
 

Further parameters e.g., ammonium, dipeptidesorganic acids, polyamines or trace elements can be added to this bundle for a more profound insight into your processes.

Please contact us for a more detailed consultation and recommendation and feel free to read our technical note on sepnt media analysis.

Related links to "Culture Media Analysis Bundle"
Available downloads:
Deliverables "Culture Media Analysis Bundle"

We will send you a short report summarizing your data within 10 business days*.

*After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5 samples.

Sample Requirements "Culture Media Analysis Bundle"

Methods are established for “state-of-the-art” chemically defined cell culture media. Due to potential matrix effects verification of sample feasibility might be necessary prior to analysis. Please note that feed media matrix should be considered separately. If you plan to measure pure feed media, feel free to contact us for more information.

Please read and sign our sample declaration sheet and prepare and ship your samples according to our sample preparation protocol.

Additional Information "Culture Media Analysis Bundle"
Amino acid Analysis Amino acid Analysis
With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our total amino acid analysis (order...
Water-soluble Vitamin Analysis Water-soluble Vitamin Analysis
Our UHPLC-MS/MS method covers the following water-soluble vitamins, vitaminoids and precursors*: Vitamin B1: Thiamine...
Trace Element Analysis Bundle for Spent Media Trace Element Analysis Bundle for Spent Media
Trace elements are determined by ICP-MS, combining highest sensitivity with excellent reliability, including: Element...
Hint!
Organic Acid Analysis - UHPLC based method Organic Acid Analysis - UHPLC based method
Organic acid content is determined using UHPLC-MS. Citric acid, Fumaric acid, Lactic acid, Malic acid, Pyruvat, Succinic acid,...
Hint!
Polyamine Analysis Polyamine Analysis
Polyamine levels in mammalian cells play a key role in cells’ viability as well as protein synthesis. Knowing the polyamine content...
Ammonium Analysis Ammonium Analysis
Assay for the determination of ammonium concentrations. The average LOQ is 0.1 mM and might vary depending on the matrix.
Dipeptide Analysis Dipeptide Analysis
UHPLC-MS/MS based measurement of dipeptides. Please contact us for more information.
Glucose and Lactate Analysis Glucose and Lactate Analysis
Enzymatic-amperometric measurement of glucose and lactate.
Analysis of Total Amino Acid Content Analysis of Total Amino Acid Content
With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our total amino acid analysis (order...
Hint!
HEK ViP NX | Medium HEK ViP NX | Medium
- Optimized virus production - best choice for transient expression in HEK cells - HEK ViP NX is a complete chemically defined...
Phosphate Analysis Phosphate Analysis
Photometric assay for the quantitative analysis of inorganic phosphate (ortho-phosphate).
Ascorbate Analysis Ascorbate Analysis
Quantitation of ascorbic acid (vitamin C) via a proprietary method by UHPLC-MS/MS.
Organic Acid Analysis - Colorimetric Assay Organic Acid Analysis - Colorimetric Assay
Organic acid content is determined using abcam colorimetric kits in combination with Thermo Fisher multimode plate reader...
Cell Studies (stability, characterization) Cell Studies (stability, characterization)
With our broad range of analytical methods (cell growth, product quantification and qualification, flow cytometry applications), we...
Process Development and Optimization Process Development and Optimization
Process development such as feed strategy optimization can take up a lot of time and resources. With our long-standing experience...
Customized Medium and Feed Development Customized Medium and Feed Development
Xell offers targeted, rapid and efficient services for the development of custom cell culture medium and feed and is your ideal...
AXP - dry powder manufacture AXP - dry powder manufacture
Accelerated Xell Production (AXP) - manufacture of customized dry powder formulations (Animal Origin Free only).  After...
HEK GM | Medium HEK GM | Medium
- Your best choice for stable expression with HEK cells - robust and effective - HEK GM is a complete chemically defined ,...
Glutathione Analysis Glutathione Analysis
UHPLC-MS/MS based measurement of glutathione (oxidized and reduced state). Please contact us for more information.
Citrate Analysis Citrate Analysis
Citrate is measured via a photometric assay.
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HEK FS | Feed HEK FS | Feed
- Unique on the marked - Ideal boost for production of viral vectors, vaccines, proteins - HEK FS is a chemically defined ,...
CHO TF | Medium CHO TF | Medium
- Perfect medium for working with transient CHO cells - growth, transfection and production - CHO TF is a complete chemically...
KONFIGURATOR
Element Analysis Bundle Element Analysis Bundle
Our ICP-MS method covers the following elemtents. Please select your elements of choice.
Customized Analytical Method Development Customized Analytical Method Development
Xell offers targeted, rapid and efficient services for the development of custom analytical methods and is your ideal partner to...
HEK ViP NB | Medium HEK ViP NB | Medium
- Optimized virus production - best choice for transient expression in HEK cells - HEK ViP NB is a complete chemically defined...
Raw Material or Supplier Evaluation Raw Material or Supplier Evaluation
Within the last few decades cell culture media has evolved from complex serum or hydrolysate containing base formulations to...
Cell Line Adaptation Cell Line Adaptation
Traditional mammalian cell culture is usually anchor-dependent and serum-supplemented, which is directly connected to a series of...
Antibody Quantification Antibody Quantification
Our HPLC method for quantification of antibodies is based on protein A affinity chromatography. If you are unsure whether your...
Endotoxin Analysis Endotoxin Analysis
The LAL (Limulus amoebocyte lysate) test is used to detect and/or quantify endotoxins from gram-negative bacterial origin. Endosafe...
Coulometric Karl-Fischer Titration Coulometric Karl-Fischer Titration
Determination of water content in solid and liquid samples using coulometric Karl-Fischer titration. Quantification range: 1 ppm...
Bioburden Testing Bioburden Testing
Determination of TAMC (total aerobic count) on TS-agar and for TYMC (total microbial count of yeasts) on SAB-agar is performed by...
Dry powder rehydration service Dry powder rehydration service
Rehydration of dry powder formulations (Animal Origin Free only). Rehydration within 10 business days after receipt of dry...
Lot-to-lot testing Lot-to-lot testing
Lot-to-lot variation is a well-known effect in media production and can occur even in chemically defined media. It can be caused...
Fat-soluble Vitamin Analysis Fat-soluble Vitamin Analysis
Our UHPLC-MS/MS method covers the following fat-soluble vitamins: Vitamin A: Retinol Vitamin D: Cholecalciferol...
AXP - liquid formulation production AXP - liquid formulation production
Accelerated Xell Production (AXP) - manufacture of customized liquid formulations (Animal Origin Free only). After production the...
TCX6D | Medium TCX6D | Medium
- Well performing medium for any CHO clone - flexible medium for stable production in batch or fed-batch processes - TCX6D...
Biosafety level 2 Analytics Biosafety level 2 Analytics
Do you have cell culture supernatants or spent medium that you would like to have analyzed, but unfortunately your samples can only...
Consulting Consulting
Whether you are just starting to set up a cell culture lab, experiencing process limitations, seeing batch-to-batch variations of...
Hint!
CHOlean | Medium CHOlean | Medium
- Your lean and robust growth medium for various applications with unmatched pricing opportunities - CHOlean Medium is a...
Medium and Feed Screenings/Benchmarkings Medium and Feed Screenings/Benchmarkings
Cell culture medium and feed selection typically involves screening and benchmarking of alternative off-the-shelf culture media...