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Analysis of Total Amino Acid Content

Analysis of Total Amino Acid Content

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  • 115
With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our... more
Product information "Analysis of Total Amino Acid Content"

With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our total amino acid analysis (order no. 115) the total amino acid content is quantified, including additional HCl hydrolysis and sample preparation step before amino acid analysis. If you are interested in the ratio of free to bound amino acids, both analyzes should be carried out.

Our proprietary, quantitative UHPLC-DAD method covers the following amino acids:

  • Alanine
  • Arginine
  • Asparagine
  • Aspartate
  • Citrulline
  • Cystine/Cysteine*
  • Glutamate
  • Glutamine
  • Glycine
  • Histidine
  • Hydroxyproline
  • Isoleucine
  • Leucine
  • Lysine
  • Methionine
  • Ornithine
  • Phenylalanine
  • Proline
  • Serine
  • Taurine
  • Threonine
  • Tryptophane
  • Tyrosine
  • Valine

 

*Cysteine rapidly oxidizes to cystine. We will provide the absolute measured concentration of cystine, and a qualitative value for cysteine if detectable.

The average LOQ is <0.03 mM, depending on amino acid and sample matrix.

 

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Deliverables "Analysis of Total Amino Acid Content"

We will send you a short report summarizing your data within 10 business days*.

*After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5 samples.

Sample Requirements "Analysis of Total Amino Acid Content"

Usually a sample amount of 1 mg (if purity is > 80%) or 1 ml is needed for each analytical service. If you are limited in your sample amount please contact us. We are open to discuss smaller volumes. If you send frozen samples for more than one analysis, it would be desirable to ship them in aliquots, to prevent repeated freezing and thawing.

Due to potential matrix effects verification of sample feasibility might be necessary prior to analysis.

For Spent Media Samples:

 

For Food, Feed and other Samples:

  • Samples can be liquid or powder. Please contact us for individual support regarding sample preparation.
  • Please read and sign our sample declaration sheet.

 

For Metabolomic Samples:

  • Samples can be cell lysates or supernatants. Please contact us for individual support regarding sample preparation.
  • Please read and sign our sample declaration sheet.
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