Technical notes

Adaptation of mammalian cell lines to serum-free culture media

Posted on 15. October 2020 by Gina Cheung

Download this note (PDF) Traditional mammalian cell culture is usually anchor-dependent and serum-supplemented. Animal-derived components tend to introduce lot-to-lot variability and blood-borne pathogens. Use of serum also tends to increase immunogenicity and production costs. Because of these issues, suspension cultures in animal-derived component free (ADCF) cell culture media are usually preferred for situations like recombinant […]

Juice is good for you – but for how long? – Testing the water soluble vitamin content of different juices over time

Posted on 30. April 2020 by Lara Riedel

Download this note (PDF) Fruit are full of vitamins and very healthy, so their juices are healthy too. That is something most people were told since they were little. We do not want to doubt that, but not all vitamins are stable, therefore the question is: Does juice age? We went to a local grocery […]

Shaken, not stirred – How to transfer suspension cultures between differently operated shaking platforms

Posted on 21. April 2020 by Lara Riedel

Download this note (PDF) Shaken cultures of suspension-adapted animal cell lines are still the method of choice for screening and small-scale culture experiments due to their easy handling, the simple and fast implementation in almost any laboratory environment and the possibility of cost-effective parallelization. However, on closer inspection, there are a few points that need […]

Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Posted on 11. January 2019 by Frederik Walter

Download this note (PDF) Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis […]

Spent media analysis – Key to optimizing cell culture processes

Posted on 28. December 2018 by Frederik Walter

Download this note (PDF) Why analyze cell culture supernatants? Different mammalian cell lines and clones exhibit distinct metabolic features. Even clones descending from the same parental cell line and expressing the same product can have individual nutritional requirements. Limitations occur when the composition of the used cell culture medium does not match the cells’ requirements. […]

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