technical-notes Archives

Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Posted on 11. January 2019 by Frederik Walter

Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis helps to monitor amino […]

Spent media analysis – Key to optimizing cell culture processes

Posted on 28. December 2018 by Frederik Walter

Why analyze cell culture supernatants? Different mammalian cell lines and clones exhibit distinct metabolic features. Even clones descending from the same parental cell line and expressing the same product can have individual nutritional requirements. Limitations occur when the composition of the used cell culture medium does not match the cells’ requirements. On the one hand, […]

Adaptation of mammalian cell lines to serum-free culture media

Posted on 25. July 2018 by Frederik Walter

The adaptation of cell lines to serum-free cell culture media and growth in suspension is not always straightforward. Learn about the do-or-die-approach and weaning and get a few helpful tips to make it a success. You might find yourself in the following situation: You have developed an adherent cell line (for example based on CHO […]

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