Technical notes

The journey to AAV production in suspension – Part 1: Scale-up your process

Posted on 7. April 2021 by Sandra Klausing

Download this note (PDF) Why should you consider transferring your AAV production from adherent to suspension? Adeno-Associated Viral vectors (AAV) are among the most widely used vectors for gene therapy applications. While cultivation systems such as roller bottles, CellSTACKs (Corning), CellFactories (Nunc, ThermoFisher Scientific), fixed bed reactors or microcarrier-based cell culture are extensively used in […]

The good and the bad impurities: Trace elements

Posted on 1. April 2021 by Anja Wuest

Download this note (PDF) Within the last few decades cell culture media has evolved from complex serum or hydrolysate containing base formulations to chemically defined, animal component-free or animal origin-free, high performance cell culture solutions. In parallel, the biopharmaceutical industry has increased its expectation regarding lot-to-lot consistency and traceability. These requirements thrust analysis of trace […]

Spent Media Analysis – The key to process optimization

Posted on 11. January 2021 by Tim Steffens

Download this note (PDF) What is the difference between cell culture media and spent media? Cell culture involves growth of cells in an artificial environment. Cell culture medium is a liquid designed to support the growth of cells in this environment. Usually, it contains a mixture of an energy source like carbohydrates in the form […]

How much protein is there in “high protein” dairy products?

Posted on 16. December 2020 by Tim Steffens

Download this note (PDF) There are many reasons to love dairy products like milk, cheese, yogurt etc. They are a powerhouse of essential nutrients and are a convenient and cost-effective source of protein. Recently grocery aisles have been flooded with different “high protein” dairy products, where extra protein is added. If you are like us, […]

Adaptation of mammalian cell lines to serum-free culture media

Posted on 15. October 2020 by Gina Cheung

Download this note (PDF) Traditional mammalian cell culture is usually anchor-dependent and serum-supplemented. Animal-derived components tend to introduce lot-to-lot variability and blood-borne pathogens. Use of serum also tends to increase immunogenicity and production costs. Because of these issues, suspension cultures in animal-derived component free (ADCF) cell culture media are usually preferred for situations like recombinant […]

Juice is good for you – but for how long? – Testing the water soluble vitamin content of different juices over time

Posted on 30. April 2020 by Tim Steffens

Download this note (PDF) Fruit are full of vitamins and very healthy, so their juices are healthy too. That is something most people were told since they were little. We do not want to doubt that, but not all vitamins are stable, therefore the question is: Does juice age? We went to a local grocery […]

Shaken, not stirred – How to transfer suspension cultures between differently operated shaking platforms

Posted on 21. April 2020 by Lara Riedel

Download this note (PDF) Shaken cultures of suspension-adapted animal cell lines are still the method of choice for screening and small-scale culture experiments due to their easy handling, the simple and fast implementation in almost any laboratory environment and the possibility of cost-effective parallelization. However, on closer inspection, there are a few points that need […]

Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Posted on 11. January 2019 by Frederik Walter

Download this note (PDF) Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis […]

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