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Transferring lab sciences into commercial products – Successful conversion of liquid prototypes of cell culture media into dry powder serves crucial benefits

Download this note (PDF) When a transfer to powder formulation is desirable and what should be considered Cell culture medium optimization is commonly carried out with the production of liquid prototypes in small scale to test concentration ranges, individual components, nutrient balances, etc. Raw materials are individually weighed out and dissolved in water sequentially or […]

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The journey to AAV production in suspension – Part 1: Scale-up your process

Download this note (PDF) Why should you consider transferring your AAV production from adherent to suspension? Adeno-Associated Viral vectors (AAV) are among the most widely used vectors for gene therapy applications. While cultivation systems such as roller bottles, CellSTACKs (Corning), CellFactories (Nunc, ThermoFisher Scientific), fixed bed reactors or microcarrier-based cell culture are extensively used in […]

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The good and the bad impurities: Trace elements

Download this note (PDF) Within the last few decades cell culture media has evolved from complex serum or hydrolysate containing base formulations to chemically defined, animal component-free or animal origin-free, high performance cell culture solutions. In parallel, the biopharmaceutical industry has increased its expectation regarding lot-to-lot consistency and traceability. These requirements thrust analysis of trace […]

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Adaptation of mammalian cell lines to serum-free culture media

Download this note (PDF) Traditional mammalian cell culture is usually anchor-dependent and serum-supplemented. Animal-derived components tend to introduce lot-to-lot variability and blood-borne pathogens. Use of serum also tends to increase immunogenicity and production costs. Because of these issues, suspension cultures in animal-derived component free (ADCF) cell culture media are usually preferred for situations like recombinant […]

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Shaken, not stirred – How to transfer suspension cultures between differently operated shaking platforms

Download this note (PDF) Shaken cultures of suspension-adapted animal cell lines are still the method of choice for screening and small-scale culture experiments due to their easy handling, the simple and fast implementation in almost any laboratory environment and the possibility of cost-effective parallelization. However, on closer inspection, there are a few points that need […]

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Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Download this note (PDF) Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis […]

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