Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis helps to monitor amino […]

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Spent media analysis – Key to optimizing cell culture processes

Why analyze cell culture supernatants? Different mammalian cell lines and clones exhibit distinct metabolic features. Even clones descending from the same parental cell line and expressing the same product can have individual nutritional requirements. Limitations occur when the composition of the used cell culture medium does not match the cells’ requirements. On the one hand, […]

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