Shaken, not stirred – How to transfer suspension cultures between differently operated shaking platforms

Download this note (PDF) Shaken cultures of suspension-adapted animal cell lines are still the method of choice for screening and small-scale culture experiments due to their easy handling, the simple and fast implementation in almost any laboratory environment and the possibility of cost-effective parallelization. However, on closer inspection, there are a few points that need […]

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Rapid Oxidation of Cysteine to Cystine in Aqueous Solutions – Implications for Spent Media Analysis

Download this note (PDF) Cysteine oxidation in cell culture media Cell culture media typically provide all the amino acids necessary for cells to grow. Limitations in one or more amino acids do not only result in suboptimal growth of cell cultures, but also negatively impact product yield and quality. During process development, spent media analysis […]

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Spent media analysis – Key to optimizing cell culture processes

Download this note (PDF) Why analyze cell culture supernatants? Different mammalian cell lines and clones exhibit distinct metabolic features. Even clones descending from the same parental cell line and expressing the same product can have individual nutritional requirements. Limitations occur when the composition of the used cell culture medium does not match the cells’ requirements. […]

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