Xell offers a range of chemically defined, high performance off-the-shelf cell culture media and feeds for various applications and cell lines, including CHO, HEK, BHK, MDXK, hybridoma and others.
All our cell culture media products are distinguished by the following characteristics:
CHO cell lines are the most prevalent mammalian expression system in the biopharmaceutical industry. Based on diverse host and production clones, Xell has developed a CHO cell culture medium and feed platform that supports highly efficient protein expression. Xell’s CHO media and feeds are developed for long-term, high performance suspension culture. They can be used in research or in manufacturing applications.
Human cell lines play a key role in transient protein expression, viral vector and vaccine production. For these applications, Xell provides HEK TF, tailored to support transfection and efficient suspension cultivation, whereas HEK GM or HEK FS further boost production. Xell’s HEK cell culture media and feeds are developed for long-term, high performance suspension culture. They can be used in research or in manufacturing applications.
The complete package for your bioprocess that speeds up your pathway to the clinic.
AXYTE cells + medium streamlines the workflow towards your production. Xell has partnered with ACYTE Biotech to combine fast stable cell line generation with highly efficient medium and feed solutions, especially tailored for the developed technology platform. Contact us or ACYTE Biotech to discuss your individual development project.
Fig. 1: Scheme of cloning procedure in semi-solid medium. (Figure provided by ACYTE Biotech)
Features of AXYTE host cells (XL-99):
Rapid cell line generation:
Features of AXYTE expression vector:
AXYTE medium and feed were developed to specifically support growth and production of AXYTE cell lines. Using several exemplary production clones, medium and feed were step-wise optimized to achieve superior performance in batch and fed-batch processes.
Features of AXYTE medium and feed:
Fig. 2: Batch (B) culture performance regarding maximum cell density (left) and maximum product titer (right) of two exemplary production clones in commercially available reference medium (Comm. Ref.) and AXYTE medium. Performance in fed-batch (FB) process with AXYTE medium and feed is shown as well.
For further information or to order our products, please contact us directly.
AXYTE cells + medium provides an accelerated pathway to the clinic and offers further savings in time to market with a shortened timeline for cell line development. The use of a rational selection strategy in semi solid phase growth allows for true clonal isolation from the start. Semi-solid phase tracking of the clones during the selection phase will assist with regulatory compliance. Using Xell’s media during cell line development makes further adaptation obsolete.
AXYTE cells + medium utilizes a process that harnesses CHO plasticity. This leads to easier selection of clones with favorable quality attributes.
Fig. 3: A set of CHO-XL99 (CHO-K1) clonal cell lines expressing antibody related products (including Fc-fusion proteins). Product concentrations were determined through a 7 day simple batch process in chemically defined conditions.
AXYTE cells + medium offers an opportunity to design an enhanced expressing cell line that meets all your needs.
Fig. 4: AXYTE clonal cell lines offer greater diversity during key stages of the clonal isolation process and process development. This leads to more robust and high performing clones.