Cell culture media and feeds

CHO cell lines are the most prevalent mammalian expression system in the biopharmaceutical industry. Based on diverse host and production clones, Xell has developed a CHO cell culture medium and feed platform that supports highly efficient protein expression. Xell’s CHO media and feeds are developed for long-term, high performance suspension culture. They can be used in research or in manufacturing applications.

• TCX6D Medium Order No. 1070
  Growth medium for various CHO cell lines.
• TCX10D Medium Order No. 1100
  Growth medium for CHO GS cell lines.
• TCX7D Feed Order No. 1080
  Advanced Supplement for CHO fedbatch processes.
• CHO TF Order No. 886
  Growth medium that supports efficient transfection of different CHO cell lines.

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Human cell lines play a key role in transient protein expression, viral vector and vaccine production. For these applications, Xell provides HEK TF, tailored to support transfection and efficient suspension cultivation, whereas HEK GM or HEK FS further boost production. Xell’s HEK cell culture media and feeds are developed for long-term, high performance suspension culture. They can be used in research or in manufacturing applications.

• HEK GM Order No. 851
  Growth and production medium for various human cell lines, e.g. HEK.
• HEK TF Order No. 861
  Growth medium that supports efficient transfection of human cell lines, e.g. HEK.
• HEK FS Order No. 871
  Feed supplement for boosting process yields of human cell cultures, e.g. HEK.

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Hybridoma cells are widely used for the development and production of monoclonal antibodies. Xell’s Hybridoma cell culture media and feeds are suitable for research and routine manufacturing.

• Hybridoma Base medium Order No. 0027B
  Growth and production medium for various hybridoma cell lines.
• Basic Feed Order No. 1092
  Feed supplement for boosting process yields of various cell cultures, e.g. hybridoma.

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• BHK Medium Order No. 910
  Growth and production medium for BHK cell lines.
• BHK FS Order No. 915
  Feed supplement for boosting process yields of BHK cell cultures.
• MDXK Medium Order No. 1010
  Growth and production medium for MDBK and MDCK cell lines.

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Fig. 1: Scheme of cloning procedure in semi-solid medium. (Figure provided by ACYTE Biotech)

Features of AXYTE host cells (XL-99):

• CHO-K1 derivative from ATCC CCL-61
• Robust, suspension adapted, low passage bank
• Exceptional growth in chemically defined conditions
• Validated cGMP cell bank fully tested to regulatory guidelines

Rapid cell line generation:

• Stringent selection and high- throughput semi-solid cloning
• State of the art cell line development facility
• DNA to clonal line (single round) in as little as 8-12 weeks

Features of AXYTE expression vector:

• Simple vector construct designed for high level expression
• Dual selection assisted expression system
• Dual expression cassette available for the insertion of mAb heavy and light chains.

AXYTE medium and feed were developed to specifically support growth and production of AXYTE cell lines. Using several exemplary production clones, medium and feed were step-wise optimized to achieve superior performance in batch and fed-batch processes.

Features of AXYTE medium and feed:

• Developed with a panel of different exemplary AXYTE production cell lines
• Medium is used during cell line generation, no further adaption needed
• Feed supplement available for high-performance fedbatch processes
• Supports high cell density, viability and product titer

Fig. 2: Batch (B) culture performance regarding maximum cell density (left) and maximum product titer (right) of two exemplary production clones in commercially available reference medium (Comm. Ref.) and AXYTE medium. Performance in fed-batch (FB) process with AXYTE medium and feed is shown as well.

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Defined monoclonality within no time

AXYTE cells + medium provides an accelerated pathway to the clinic and offers further savings in time to market with a shortened timeline for cell line development. The use of a rational selection strategy in semi solid phase growth allows for true clonal isolation from the start. Semi-solid phase tracking of the clones during the selection phase will assist with regulatory compliance. Using Xell’s media during cell line development makes further adaptation obsolete.

Greater diversity during selection

AXYTE cells + medium utilizes a process that harnesses CHO plasticity. This leads to easier selection of clones with favorable quality attributes.

Fig. 3: A set of CHO-XL99 (CHO-K1) clonal cell lines expressing antibody related products (including Fc-fusion proteins). Product concentrations were determined through a 7 day simple batch process in chemically defined conditions.

Superior expression

AXYTE cells + medium offers an opportunity to design an enhanced expressing cell line that meets all your needs.

Fig. 4: AXYTE clonal cell lines offer greater diversity during key stages of the clonal isolation process and process development. This leads to more robust and high performing clones.