Today, we are delighted to share our latest R&D findings with you: Our straightforward approach to easily upscale a process for AAV8 production in HEK 293 cells cultivated in our chemically defined, animal component free and protein free HEK ViP NX medium.
Starting with a shake flask process, we applied and successfully transferred our easy, non-optimized suspension culture protocol to a controlled bioreactor scale which resulted in increased AAV8 genomic titers. This is just another story that proves the potency of our HEK ViP product series.
Figure: Comparison of AAV8 genomic titers in supernatants of HEK293 cells cultivated in Xell’s HEK ViP NX medium. Cultivation in bioreactor leads to increased genomic titer in culture supernatant.
Straight forward approach:
HEK 293 cells cultivated in HEK ViP NX medium were transfected applying a non-optimized protocol in a shake flask or benchtop bioreactor scale at cell densities of about 2 million cells/mL. PEI MAX™ (Polysciences) was used as a transfection reagent and a 2-plasmid system was introduced (4:1 ratio; precomplexing in HEK ViP NX medium) to produce AAV8.