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CHOlean | Medium Sample

CHOlean | Medium Sample

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  • 1140-0001-S
CHOlean Medium is a complete chemically defined , animal-component–free, protein-free, and... more
Product information "CHOlean | Medium Sample"

CHOlean Medium is a complete chemically defined, animal-component–free, protein-free, and ready-to-use cell culture medium. It has a lean medium formulation designed for batch and fed-batch processes with different CHO cell lines. CHOlean is developed with a minimal set of well-balanced components to achieve a lean, robust and flexible medium basis. In addition, it is capable of competing with state-of-the-art media for highest cell growth and product titer. The formulation is suitable for a broad range of different CHO cell lines, including K1 and DG44. Furthermore, CHOlean is optimized for easy adaptation from other media and is a robust platform to build high-performance fed-batch processes on. The medium supports cell growth and production of recombinant proteins and antibodies in suspension culture. It can be used in research or in manufacturing applications.

  • For CHO cell lines
  • Chemically defined, serum-free, animal component-free and hydrolysate-free
  • Little or no adaptation required from other serum-free media
  • Supports stable growth of suspension cells at high viability in seed train culture
  • w/o L-glutamine
  • w/o HT (hypoxanthine/thymidine)
  • Appoved by ISO 9001 QMS

 CHOlean-Performance

Figure 1: Relative titers (bars) and maximum viable cell densities (VCD, rhombs) of two CHO cell lines in CHOlean compared to current state-of-the-art, chemically defined, ADCF expression media for CHO suspension cells of five different competitors.

 

CHOlean-Number-of-Components

Figure 2: Number of components of different classes (amino acids, vitamins, salts, buffer, iron and others) of CHOlean compared to the basal formulation DMEM/Ham's F12 as well as the comparison of total number of components of CHOlean, DMEM/Ham's F12 and some state-of-the-art production media.

 

 

 

 

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