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qPCR-based Analysis

qPCR-based Analysis

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Analysis via qPCR (quantitative polymerase chain reaction) is extremely sensitive and can be... more
Product information "qPCR-based Analysis"

Analysis via qPCR (quantitative polymerase chain reaction) is extremely sensitive and can be utilized for detection of target DNA/RNA sequences from various sources. Aapplications range from transcript quantification to genomic virus titer determination (AAV, lentivirus, etc.). Quantification of virus titers is an essential tool for gene therapy applications e.g., when it comes to up-scaling AAV production process. Fell free to read our technical note on up-scaling AAV production processes and find out how we succeeded in increasing AAV-2 and AAV-8 genomic titers by transferring processes from shake flasks to bioreactors:

 AAV-2-Genomic-titers

Please inquire to find out more about our qPCR analysis services and prices.

 

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Sample Requirements "qPCR-based Analysis"

Samples can be e.g. cell culture supernatants or pellets.

Please read and sign our  sample declaration sheet and contact us regarding sample preparation.

KONFIGURATOR
Element Analysis Bundle Element Analysis Bundle
Our ICP-MS method covers the following elemtents. Please select your elements of choice.
ELISA-based Analysis ELISA-based Analysis
As an immunological assay, the ELISA (enzyme-linked immunosorbent assay) can be applied for a broad range of cell culture derived...
Customized Analytical Method Development Customized Analytical Method Development
Xell offers targeted, rapid and efficient services for the development of custom analytical methods and is your ideal partner to...
Process Development and Optimization Process Development and Optimization
Process development such as feed strategy optimization can take up a lot of time and resources. With our long-standing experience...
Culture Media Analysis Bundle Culture Media Analysis Bundle
Spent media analysis is the analysis of the used media over the course of the production process and facilitates the selection of...
ddPCR-based Analysis ddPCR-based Analysis
The digital droplet PCR (ddPCR) is a method suitable for absolute quantification of DNA and RNA samples without the need of...
Raw Material or Supplier Evaluation Raw Material or Supplier Evaluation
Within the last few decades cell culture media has evolved from complex serum or hydrolysate containing base formulations to...
Antibody Quantification Antibody Quantification
Our HPLC method for quantification of antibodies is based on protein A affinity chromatography. If you are unsure whether your...
Particle Size Distribution Particle Size Distribution
Determination of the particle size distribution in solid samples using Laser diffraction plus advanced Polarization Intensity...
ADCC Reporter Bioassay ADCC Reporter Bioassay
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of cell-mediated immune response, initiated by specific...
Trace Element Analysis Bundle for Spent Media Trace Element Analysis Bundle for Spent Media
Trace elements are determined by ICP-MS, combining highest sensitivity with excellent reliability, including: Element...
Hint!
Organic Acid Analysis - UHPLC based method Organic Acid Analysis - UHPLC based method
Organic acid content is determined using UHPLC-MS. Citric acid, Fumaric acid, Lactic acid, Malic acid, Pyruvat, Succinic acid,...
Bioburden Testing Bioburden Testing
Determination of TAMC (total aerobic count) on TS-agar and for TYMC (total microbial count of yeasts) on SAB-agar is performed by...
Cell Studies (stability, characterization) Cell Studies (stability, characterization)
With our broad range of analytical methods (cell growth, product quantification and qualification, flow cytometry applications), we...
Hint!
HEK ViP NX | Medium HEK ViP NX | Medium
- Optimized virus production - best choice for transient expression in HEK cells - HEK ViP NX is a complete chemically defined...
AXP - dry powder manufacture AXP - dry powder manufacture
Accelerated Xell Production (AXP) - manufacture of customized dry powder formulations (Animal Origin Free only).  After...
Hint!
Polyamine Analysis Polyamine Analysis
Polyamine levels in mammalian cells play a key role in cells’ viability as well as protein synthesis. Knowing the polyamine content...
Dipeptide Analysis Dipeptide Analysis
UHPLC-MS/MS based measurement of dipeptides. Please contact us for more information.
Cell Line Adaptation Cell Line Adaptation
Traditional mammalian cell culture is usually anchor-dependent and serum-supplemented, which is directly connected to a series of...
Water-soluble Vitamin Analysis Water-soluble Vitamin Analysis
Our UHPLC-MS/MS method covers the following water-soluble vitamins, vitaminoids and precursors*: Vitamin B1: Thiamine...
Amino acid Analysis Amino acid Analysis
With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our total amino acid analysis (order...
Glutathione Analysis Glutathione Analysis
UHPLC-MS/MS based measurement of glutathione (oxidized and reduced state). Please contact us for more information.
HEK ViP NB | Medium HEK ViP NB | Medium
- Optimized virus production - best choice for transient expression in HEK cells - HEK ViP NB is a complete chemically defined...
Lot-to-lot testing Lot-to-lot testing
Lot-to-lot variation is a well-known effect in media production and can occur even in chemically defined media. It can be caused...
Customized Medium and Feed Development Customized Medium and Feed Development
Xell offers targeted, rapid and efficient services for the development of custom cell culture medium and feed and is your ideal...
AXP - liquid formulation production AXP - liquid formulation production
Accelerated Xell Production (AXP) - manufacture of customized liquid formulations (Animal Origin Free only). After production the...
Analysis of Total Amino Acid Content Analysis of Total Amino Acid Content
With our amino acid measurement (order no. 103) free amino acids are measured, whereas with our total amino acid analysis (order...
HEK Media Bundle HEK Media Bundle
Our HEK Media Bundle is the easiest approach for you to test, identify and select the best matching serum-free,...
Highly abundant Cation Analysis Highly abundant Cation Analysis
Measurement of the highly abundant cations Sodium, Potassium, Magnesium, Calcium and Iron using ICP-MS.
Manufacturing Science Manufacturing Science
You have developed your own product, but you face challenges in context of manufacturing? Make it easy for yourself, save time and...
Formulation Development Formulation Development
Do you need help formulating your own medium, supplement or another component? Or do you lack capacities? We can support you with:...
Consulting Consulting
Whether you are just starting to set up a cell culture lab, experiencing process limitations, seeing batch-to-batch variations of...
Citrate Analysis Citrate Analysis
Citrate is measured via a photometric assay.
Medium and Feed Screenings/Benchmarkings Medium and Feed Screenings/Benchmarkings
Cell culture medium and feed selection typically involves screening and benchmarking of alternative off-the-shelf culture media...
Ammonium Analysis Ammonium Analysis
Assay for the determination of ammonium concentrations. The average LOQ is 0.1 mM and might vary depending on the matrix.
Ascorbate Analysis Ascorbate Analysis
Quantitation of ascorbic acid (vitamin C) via a proprietary method by UHPLC-MS/MS.
HEK TF | Medium HEK TF | Medium
- Well established transfection medium - ideal for production of recombinant proteins and antibodies - HEK TF is a complete...
Qualitative Peptide Size Determination using SEC Qualitative Peptide Size Determination using SEC
Qualitative determination of peptide sizes using size exclusion chromatography (SEC). SEC separates polymer molecules based on...
HEK TF SILAC | Medium HEK TF SILAC | Medium
HEK TF SILAC is a complete chemically defined , animal-component-free medium variant without arginine and lysine. Therefore it can...
Pyridoxal Phosphate Pyridoxal Phosphate
Measurement of Pyridoxal Phosphate using UHPLC-MS/MS.